TABLE 3.

Metabolism of cyclo-d4G in CEM and PBM cells

Cell type	Time (h)	Cellular concentration (pmol/106 cells)a:
		Sugar catabolitesb	d4G	Cyclo-d4G	Cyclo-d4G-MPd	d4G-MP	d4G-DPc	d4G-TPe
CEM	1	0.41 ± 0.11	0.54 ± 0.26	7.31 ± 1.48	0.55 ± 0.34	0.26 ± 0.06	0.31 ± 0.10	0.15c
	2	0.36 ± 0.05	0.36 ± 0.19	3.32 ± 0.81	0.15 ± 0.08	0.08 ± 0.03	0.23 ± 0.13	0.09 ± 0.04
	4	0.60 ± 0.12	1.39 ± 0.06	4.40 ± 0.38	0.27 ± 0.07	0.16 ± 0.03	0.26 ± 0.02	0.18 ± 0.01
	8	0.63 ± 0.04	2.40 ± 0.21	4.65 ± 0.95	0.29 ± 0.15	0.28 ± 0.02	0.29 ± 0.05	0.17 ± 0.02
	24	—c	1.91c	3.46 ± 0.25	0.22 ± 0.07	0.92 ± 0.17	0.36 ± 0.04	0.41 ± 0.19
PBM	1	0.07 ± 0.01	0.12 ± 0.02	0.71 ± 0.18	0.13 ± 0.04	0.06 ± 0.01	0.13 ± 0.08	0.11 ± 0.05
	2	0.09 ± 0.05	0.19 ± 0.04	0.67 ± 0.26	0.10 ± 0.04	0.04 ± 0.01	0.08 ± 0.01	0.08 ± 0.03
	4	0.10 ± 0.03	0.27 ± 0.08	1.3 ± 0.17	0.10 ± 0.01	0.12 ± 0.10	0.11 ± 0.02	0.07 ± 0.01
	8	0.16 ± 0.02	0.92 ± 0.38	1.48 ± 0.18	0.07 ± 0.01	0.08 ± 0.01	0.15 ± 0.01	0.09 ± 0.01
	24	0.63 ± 0.07	1.87 ± 0.35	0.36 ± 0.07	0.30 ± 0.06	0.12 ± 0.09	0.10 ± 0.02	0.05 ± 0.02

^aValues represent means±SD of three independent experiments.

^bThree distinct peaks for sugar metabolites separated by HPLC.

^cSamples lost or affected by poor chromatographic resolution.

^dIdentity inferred from relative retention time.

^eRetention time estimated using a ddGTP standard.