FIG. 5.

Analysis of bradyzoite stage conversion. HFF monolayers were infected for 2 h with T. gondii, washed to remove extracellular parasites, and treated either for 6 days with 10 μg/ml of aureobasidin A (AbA) or for 4 days with alkaline medium and ambient CO₂ (pH 8.1). After cell fixation in 4% paraformaldehyde and permeabilization in 0.2% Triton X-100, intracellular parasites were visualized with an antiserum against T. gondii tachyzoites, followed by fluorescein-conjugated secondary antibodies (α-T. gondii), or with an antiserum raised against the bradyzoite-specific antigen BAG5, followed by Cy3-conjugated secondary antibodies (α-BAG5). Bar = 5 μm.