TABLE 2.
Primers used in this study
| Primer | Sequence (5′-3′) | Nucleotide positions corresponding to the insert of pBK-LHK-5 shown in Fig. 1 | Purpose |
|---|---|---|---|
| LPW606 | GGCAGGCAGCAACCATTC | 673 to 690 | Sequencing of insert of pBK-LHK-5 |
| LPW608 | ATTGACCTGTCTGCACCG | 1412 to 1429 | Sequencing of insert of pBK-LHK-5 and ampC gene |
| LPW621 | GGCTATCTGTTGCAGCAACC | 19 to 38 | PCR and sequencing of ampR gene |
| LPW622 | CGACCAGTGCAGTAAACGGT | 1385 to 1366 | PCR and sequencing of ampR gene and PCR for preparation of the 298-bp blaLHK-5 probe |
| LPW663 | CGGATTACCCCATTTTCCCG | 1088 to 1107 | PCR and sequencing of ampC gene and PCR for preparation of the 298-bp blaLHK-5 probe |
| LPW664 | CATGTCTGCCCGGAATGTGC | 2405 to 2386 | PCR and sequencing of ampC gene |
| LPW665 | CAATCTAACGAGGGCTGAGT | −118 to −99 | PCR and sequencing of ampR gene |
| LPW679 | TGTTCGACTCGTCGATAGGC | 239 to 220 | PCR and sequencing of ampR gene |
| LPW697 | ATTGCGGTCGGCATTACAGC | 1906 to 1925 | Sequencing of ampC gene |
| LPW1946 | GATCAAAGGCCTGGCTGGC | 878 to 896 | PCR of ampC-ampR gene for construction of recombinant plasmid pLHKCR |
| LPW1947 | GGTCGAACCATCGTGAACC | −2 to 17 | PCR of ampC gene for construction of recombinant plasmid pLHKC |
| LPW1948 | ATGTCTGCCCGGAATGTGC | 2405 to 2387 | PCR of ampC and ampC-ampR genes for construction of recombinant plasmids pLHKC and pLHKCR |