TABLE 1.
Results of PDI experimentsa
| Light dose (J · cm−2) | % Survival (SD) | TriP[4] fluorescence pattern | Electron microscopy
|
|
|---|---|---|---|---|
| e face | p face | |||
| 0 | 70.3 (47.6) | Peripheral circular | ||
| 1.8 | 46.2 (46.2) | Peripheral circular | Fewer IMPs, rectangular areas without structure, phase separation | Patches without structure |
| 5.4 | 0.20 (0.09) | Peripheral circular or cyto- plasmic without a vacuole | Fewer IMPs, rectangular areas without structure are larger, phase separation | Fewer IMPs, phase separation, more patches without structure, blebbing |
| 12.6 | No survival detectable | Cytoplasmic without a vacuole | No IMPs, rectangular areas have merged | Less IMPs, phase separation, more patches without structure, blebbing |
a
The incubation time was 1 min. The results are for confocal and electron microscopy images of C. albicans cells diluted in PBS and illuminated in the presence of 25 μM TriP[4]. The fluence rate was 30 mW · cm−2.