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. 2005 May;49(5):1823–1829. doi: 10.1128/AAC.49.5.1823-1829.2005

FIG. 1.

FIG. 1.

RFLP analysis of class 1 and class 2 integrons. (A) AluI digest of class 1 integron DNA from E. coli isolates: lanes 1, 20, and 28, DNA molecular weight marker λ-EcoT14I/BglII digest (Takara Bio Inc.); lanes 2, 19, 21, and 27, DNA molecular weight marker III (Roche Diagnostics); anes 3, 4, 6, 8 to 13, 17, 18, and 25, type I1 (aadA1); lane 7, type III1 (dhfrA12-orfF-aadA2); lanes 14, 15, and 26, type II1 (dhfrA1-aadA1); lanes 16, 23, and 24, type IV1 (dhfrA16-aadA2); lane 22, type V1 (aadA2). (B) HaeII digest of class 2 integron DNA from E. coli isolates: lane 1, DNA molecular weight marker λ-EcoT14I/BglII digest (Takara Bio Inc.); lane 2, DNA molecular weight marker III (Roche Diagnostics); lanes 3 to 10, 12, 13, and 15 to 17, type II2 (dhfrA1-sat-aadA1-orfX); lanes 11 and 14, type I2 (sat-aadA1-orfX); lane 18, type III2 (orf-sat-aadA1-orfX).