TABLE 1.
Study strains and plasmids
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| S. aureus strains | ||
| NCTC 8325-4 | Commonly utilized laboratory strain cured of known prophages | 28 |
| RN4220 | NCTC 8325-4 r-; capable of stably maintaining recombinant plasmids | 19 |
| SA-K1712 | NCTC 8325-4 with norA disrupted by tetK | 16 |
| SA-K1748 | MDR mutant of SA-K1712; GrlA S80F | 16 |
| SA-K1748-P | SA-K1748 + pALC2073 | This study |
| SA-K1748-R | SA-K1748 + pK434 | This study |
| SA-K1748 mepA- | mepA disrupted by fusion with lacZ | This study |
| SA-K2068 | MDR mutant of NCTC 8325-4 | 15 |
| SA-K2068-P | SA-K2068 + pALC2073 | This study |
| SA-K2068-R | SA-K2068 + pK434 | This study |
| SA-K2068 mepA- | mepA disrupted by fusion with lacZ | This study |
| SA-K1758 | NCTC 8325-4 with norA deleted | 31 |
| SA-K1758-P | SA-K1758 + pALC2073 | This study |
| SA-K1758-A | SA-K1758 + pK436 | This study |
| SA-K1758-B | SA-K1758 + pK438 | This study |
| SA-K1758-AB | SA-K1758 + pK440 | This study |
| Plasmids | ||
| pALC2073 | S. aureus vector containing a tetracycline-inducible promoter controlling expression of cloned genes, Cmr | 1 |
| pAZ106 | Suicide vector used to disrupt mepA | 17 |
| pK434 | pALC2073-mepR | This study |
| pK436 | pALC2073-mepA | This study |
| pK438 | pALC2073-mepB | This study |
| pK440 | pALC2073-mepAB | This study |