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. 2023 Dec 30;130(4):568–584. doi: 10.1038/s41416-023-02553-y

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© The Author(s) 2023, corrected publication 2024

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Fig. 2 — a Box plots showing the intracellular levels of 3-PG, serine, glycine, GSH, UTP and TTP in in a pannel of 5 NSCLC cell lines 24 h after 6 Gy irradiation. Unpaired 2-tailed t-test was used. b Fold change of mean fluorescence intensity (MFI) of SHMT2 protein expression using flow cytometry in NSCLC cell lines (n = 6) in response to irradiation, 4 Gy and 6 Gy for 6 and 24 h. An unpaired 2-tailed t test was used to compare irradiated cells vs control cells. c Flow cytometry graphs of SHMT2 protein expression in NSCLC and cell lines in response to irradiation, 4 Gy and 6 Gy for 24 h. d Fold change of SHMT2 protein expression measured by immunoblot in n = 6 NSCLC cell lines 24 h post-irradiation and n = 2 biological replicate for each cell line. An unpaired 2-tailed t test has been used to compare irradiated cells and control cells. e Cell growth curves showing the confluence (%) of A549 control cells and A549 cells with overexpression of NKX2-1 upon exposure to 4 Gy irradiation. Fold change of area under the curve (AUC) of the A549 control NKX2-1 overexpression cell growth curves. Each dot represents an independent experiment. Unpaired 2-tailed t-test has been performed. Statistical analysis *p-value < 0.05, **p-value < 0.01, ****p-value < 0.0001.