FIG. 2.

Effect of nerolidol on the survival of L. amazonensis. The cell viability of promastigotes (A) and amastigotes (B) was evaluated after the indicated periods of incubation with different concentrations of nerolidol. The growth of treated cultures is shown as the percentage of control parasites incubated in medium alone. (C) Effect of nerolidol on intracellular amastigotes. Macrophages were infected with L. amazonensis promastigotes for 3 h. After infection, nerolidol was added at final concentrations of 50, 75, or 100 μM for 48 h. Cells were fixed and stained, and the percentage of infected macrophages was determined by counting at least 100 cells/coverslip in triplicate coverslips. Cultures were tested in triplicates, and the results shown are the average of at least three independent experiments.