FIG. 6.
HPLC analysis of [3H]FPP-labeled L. amazonensis promastigotes. Labeled parasite extracts were analyzed by RP-HPLC by using methanol-water-hexane-propan-2-ol-methanol as a solvent system. The retention times of coinjected standards are indicated by arrows: G, geraniol; F, farnesol; Pn, prenols of “n” isoprene units; D55 and D60, dolichols with 55 and 60 C; erg, ergosterol. Fractions of 0.5 ml (0.5 min) were collected and monitored for radioactivity. (A) Control; (B) promastigotes treated with 30 μM nerolidol. Identical numbers of cells were used to prepare the extracts analyzed in panels A and B.