TABLE 3.
Antimicrobial susceptibilities of the clinical isolates; their E. coli A15 transconjugants; and the TEM-85-, TEM-86-, TEM-93-, and TEM-94-producing E. coli DH5α transformants
| Strain no. and ESBL variant | MIC (μg/ml)a
|
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AMP | PIP | TZP | CEF | CTX | CTX + CLA | CAZ | CAZ + CLA | ATM | ATM + CLA | FOX | IPM | AMK | GEN | |
| K. pneumoniae SU2949/98 TEM-85 | >512 | >512 | 128 | 64 | 2 | 0.5 | 512 | 2 | 128 | 0.5 | 32 | 0.125 | 256 | 64 |
| K. pneumoniae SU3220/98 TEM-85 | >512 | >512 | 4 | 32 | 1 | 0.06 | 512 | 2 | 256 | 0.25 | 4 | 0.125 | 256 | 128 |
| K. pneumoniae SU3519/98 TEM-85 | >512 | >512 | 8 | 64 | 4 | 0.06 | >512 | 4 | 512 | 1 | 4 | 0.125 | 256 | 256 |
| E. coli SU3408/98 TEM-85 | >512 | 256 | ≤0.5 | 16 | 1 | ≤0.03 | 64 | 0.25 | 32 | 0.06 | 4 | 0.25 | 128 | 128 |
| K. pneumoniae SU2946/98 TEM-86 | >512 | 128 | 4 | 256 | 4 | 0.06 | 128 | 1 | 16 | 0.125 | 4 | 0.125 | 256 | 64 |
| E. coli SU2947/98 TEM-29 | 256 | 256 | 16 | 32 | 0.25 | 0.06 | 4 | 0.125 | 0.25 | 0.06 | 2 | 0.25 | 2 | 1 |
| K. pneumoniae GD3658/98 TEM-47 | >512 | >512 | 32 | >512 | 8 | 0.25 | 128 | 1 | 128 | 0.125 | 8 | 0.125 | 1 | 32 |
| K. pneumoniae GD3661/98 TEM-47 | >512 | >512 | 4 | >512 | 32 | 0.25 | 256 | 1 | 512 | 0.25 | 32 | 0.125 | 1 | 32 |
| K. pneumoniae GD3670/98 TEM-47 | >512 | >512 | 16 | >512 | 16 | 0.25 | 128 | 1 | 128 | 0.125 | 32 | 0.125 | 1 | 64 |
| E. coli GD3667/98 TEM-47 | >512 | 256 | ≤0.5 | 256 | 8 | ≤0.03 | 16 | 0.125 | 16 | ≤0.03 | 2 | 0.125 | 2 | 128 |
| E. coli GD3657/98 TEM-94 | >512 | 256 | 1 | >512 | 32 | 0.06 | 32 | 0.25 | 16 | 0.06 | 8 | 0.125 | 64 | 256 |
| E. coli GD3668/98 TEM-94 | >512 | >512 | 2 | >512 | 128 | 1 | 64 | 1 | 64 | 0.5 | 32 | 1 | 16 | 32 |
| E. coli KR 3445/98 TEM-93 | >512 | 256 | 2 | 512 | 4 | 0.06 | 64 | 0.25 | 64 | 0.06 | 8 | 0.125 | 1 | 0.5 |
| E. coli KR 3447/98 TEM-93 | >512 | 512 | 2 | 512 | 8 | 0.06 | 64 | 0.5 | 64 | 0.06 | 8 | 0.125 | 1 | 0.5 |
| K. pneumoniae CZ 9455/99 TEM-4 (+CTX-M-3)b | >512 | >512 | 32 | >512 | 256 | 2 | 64 | 2 | 64 | 0.5 | 128 | 0.125 | >256 | >256 |
| K. pneumoniae CZ 9459/99 TEM-4 (+CTX-M-3)b | >512 | >512 | 64 | >512 | 256 | 2 | 64 | 2 | 64 | 0.5 | 64 | 0.25 | >256 | >256 |
| K. oxytoca BB 1753/98 | >512 | 512 | 1 | >512 | 64 | 0.06 | 4 | 0.125 | 4 | 0.125 | 64 | 0.25 | >256 | >256 |
| TEM-4 (+CTX-M-3) | ||||||||||||||
| R+ (E. coli SU3408/98) TEM-85 | >512 | 128 | ≤0.5 | 8 | 0.25 | ≤0.03 | 64 | 0.25 | 16 | 0.06 | 4 | 0.5 | 1 | 16 |
| R+ (K. pneumoniae GD3661/98, GD3670/98, and GD3667/98) TEM-47 | >512 | 64-128 | ≤0.5 | 128 | 1-2 | ≤0.03 | 16-32 | 0.125 | 8-16 | ≤0.03 | 1-2 | 0.125 | 0.5-1 | 16-32 |
| R+ (E. coli GD3657/98 and GD3668/ 98) TEM-94 | >512 | 128 | ≤0.5 | 128 | 4 | ≤0.03 | 8-16 | 0.125 | 2 | ≤0.03 | 1 | 0.125 | 4 | 32 |
| R+ (E. coli KR3445/98) TEM-93 | >512 | 256 | ≤0.5 | 256 | 2 | ≤0.03 | 32 | 0.125 | 16 | ≤0.03 | 2 | 0.25 | 0.5 | 0.5 |
| R+ (K. pneumoniae CZ9455/99 and CZ9459/99) TEM-4 | >512 | 64-128 | 1-2 | 64-128 | 4-8 | ≤0.03 | 4 | 0.06 | 2-4 | ≤0.03 | 2 | 0.125 | 16-32 | 64 |
| R+ (K. oxytoca BB1753/98) TEM-4 | >512 | 64 | 1 | 128 | 4 | ≤0.03 | 4 | 0.125 | 2 | ≤0.03 | 2 | 0.125 | >256 | >256 |
| E. coli DH5α(pGBT-85) TEM-85 | >512 | 128 | ≤0.5 | 16 | 2 | ≤0.03 | 256 | 0.25 | 128 | 0.25 | 2 | 0.25 | ND | ND |
| E. coli DH5α(pGBT-86) TEM-86 | >512 | 32 | ≤0.5 | 256 | 4 | ≤0.03 | 64 | 0.25 | 8 | 0.125 | 2 | 0.25 | ND | ND |
| E. coli DH5α(pGBT-93) TEM-93 | >512 | 128 | ≤0.5 | 128 | 2 | ≤0.03 | 8 | 0.06 | 8 | ≤0.03 | 2 | 0.125 | ND | ND |
| E. coli DH5α(pGBT-94) TEM-94 | >512 | 128 | ≤0.5 | 128 | 4 | ≤0.03 | 8 | 0.06 | 4 | ≤0.03 | 2 | 0.125 | ND | ND |
| E. coli A15 R− | 2 | ≤0.5 | ≤0.5 | 2 | ≤0.03 | ≤0.03 | 0.125 | 0.125 | 0.06 | ≤0.03 | 1 | 0.125 | 0.5 | 0.25 |
| E. coli DH5α | 2 | ≤0.5 | ≤0.5 | 2 | ≤0.03 | ≤0.03 | 0.125 | 0.06 | 0.06 | ≤0.03 | 2 | 0.125 | ND | ND |
| E. coli ATCC 25922 | 4 | 2 | 2 | 8 | 0.06 | ≤0.03 | 0.25 | 0.125 | 0.06 | 0.06 | 4 | 0.125 | 1 | 0.5 |
a
Abbreviations: AMP, ampicillin; PIP, piperacillin; TZP, piperacillin with tazobactam; CEF, cephalothin; CTX, cefotaxime; CLA, clavulanate; CAZ, ceftazidime; ATM, aztreonam; FOX, cefoxitin; IPM, imipenem; AMK, amikacin; GEN, gentamicin; ND, not determined; R+, transconjugants; R−, E. coli strain without plasmids, used as a recipient in mating.
b
Some of the MICs for these isolates were published previously (4).