Final trophoblast differentiation medium (BAP)
| Reagent | Final concentration | Amount |
|---|---|---|
| BMP4 (10 μg/mL) | 10 ng/mL | 10 μL |
| A83-01 (1 mM) | 1 μM | 10 μL |
| PD173074 (0.1 mM) | 0.1 μM | 10 μL |
| TeSR1 medium (without FGF-2) | N/A | q.s. 10 mL |
| Total | N/A | 10 mL |
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