Fixative	Incubation time
0.4% formaldehyde in HBSS	10–15 min at 18°C–23°C
‒20°C 100% acetone	5 min at 18°C–23°C
‒20°C 100% methanol	10–15 min at 18°C–23°C
‒20°C 95% methanol, 5% acetic acid	5–10 min at 18°C–23°C