Figure 3.

MTs induce phase separation of CPC. About 100 nM of GMpCpp-stabilized rhodamine-labeled MTs induced phase separation of 1 μM soluble GFP-CPC as imaged by TIRF microscopy; scale bar for gray scale images represent 5 μm; scale bar for pseudo-colored inset represents 1 μm. The top panel shows soluble GFP-CPC/CPC sample; middle panel shows CPC phase separation induced by PEG; the bottom panel shows CPC phase separation induced by MTs (conditions without PEG). For reference, image to the right shows GMpCpp-stabilized rhodamine-labeled single MTs (not bundled) before the incubation with the CPC; scale bar represents 5 μm. Experiment was repeated twice. CPC, chromosomal passenger complex; MT, microtubule; TIRF, total internal reflection fluorescence.