Table 2.
Steps of the Seahorse assay.
Phase | Measured parameter | Pharmaceutical | Number of cycles |
---|---|---|---|
1 | Basal respiration | 10 | |
2 | ATP-coupled respiration | Oligomycin | 18 |
3 | Maximal respiration | FCCP | 8 |
4 | Non-mitochondrial respiration | Sodium azide | 24 |