Table 1.
Plackett-Burman design matrix to screen beneficial parameters for S. baarnensis MH-133 antibacterial metabolite synthesis.
| Run no. | pH | Incubation time (day) | Cultivation method (rpm) | Inoculum size (V/V) | Temperature (°C) | Magnesium chloride (g/l) | Casein (g/l) | Yeast extract (g/l) | Starch (g/l) | Elicitation (V/V) | NaCl (g/l) | inhibition zone diameter (mm) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 9 | 12 | 0 | 4 | 25 | 13.2 | 0.5 | 3 | 15 | 6 | 15 | 16.5 |
| 2 | 7 | 12 | 200 | 8 | 25 | 4.4 | 0.5 | 3 | 5 | 10 | 15 | 9.5 |
| 3 | 7 | 12 | 200 | 4 | 35 | 13.2 | 1.5 | 1 | 5 | 6 | 15 | 13 |
| 4 | 9 | 4 | 200 | 8 | 25 | 13.2 | 1.5 | 3 | 5 | 6 | 5 | 16.5 |
| 5 | 9 | 12 | 200 | 4 | 25 | 4.4 | 1.5 | 1 | 15 | 10 | 5 | 0 |
| 6 | 7 | 4 | 0 | 8 | 25 | 13.2 | 1.5 | 1 | 15 | 10 | 15 | 0 |
| 7 | 7 | 4 | 200 | 4 | 35 | 13.2 | 0.5 | 3 | 15 | 10 | 5 | 0 |
| 8 | 7 | 4 | 0 | 4 | 25 | 4.4 | 0.5 | 1 | 5 | 6 | 5 | 9.5 |
| 9 | 9 | 4 | 200 | 8 | 35 | 4.4 | 0.5 | 1 | 15 | 6 | 15 | 13.5 |
| 10 | 9 | 12 | 0 | 8 | 35 | 13.2 | 0.5 | 1 | 5 | 10 | 5 | 0 |
| 11 | 9 | 4 | 0 | 4 | 35 | 4.4 | 1.5 | 3 | 5 | 10 | 15 | 0 |
| 12 | 7 | 12 | 0 | 8 | 35 | 4.4 | 1.5 | 3 | 15 | 6 | 5 | 21 |