Table 2.
BBD for optimization of the factors affecting the production of antibacterial metabolites by S. baarnensis MH-133.
| Run Order | Incubation time (day) | Inoculum size % (v/v) | Elicitation % (v/v) | yeast extract (g/l) | Inhibition zone diameter (mm) |
|---|---|---|---|---|---|
| 1 | 4 | 8 | 8 | 2 | 13 |
| 2 | 12 | 8 | 8 | 2 | 21 |
| 3 | 4 | 6 | 6 | 2 | 12 |
| 4 | 12 | 6 | 8 | 1 | 14 |
| 5 | 4 | 6 | 8 | 1 | 10 |
| 6 | 8 | 6 | 8 | 2 | 16 |
| 7 | 8 | 6 | 8 | 2 | 15 |
| 8 | 4 | 4 | 8 | 2 | 11 |
| 9 | 8 | 6 | 6 | 1 | 13 |
| 10 | 12 | 4 | 8 | 2 | 17 |
| 11 | 8 | 4 | 8 | 3 | 14 |
| 12 | 8 | 4 | 8 | 1 | 12 |
| 13 | 12 | 6 | 10 | 2 | 17 |
| 14 | 8 | 8 | 8 | 3 | 18 |
| 15 | 8 | 8 | 8 | 1 | 13 |
| 16 | 8 | 6 | 10 | 1 | 14 |
| 17 | 12 | 6 | 6 | 2 | 21 |
| 18 | 4 | 6 | 8 | 3 | 13 |
| 19 | 4 | 6 | 10 | 2 | 11 |
| 20 | 8 | 4 | 6 | 2 | 16 |
| 21 | 8 | 8 | 6 | 2 | 20 |
| 22 | 8 | 6 | 6 | 3 | 23 |
| 23 | 8 | 4 | 10 | 2 | 10 |
| 24 | 8 | 6 | 8 | 2 | 16 |
| 25 | 8 | 6 | 10 | 3 | 12 |
| 26 | 8 | 8 | 10 | 2 | 14 |
| 27 | 12 | 6 | 8 | 3 | 21 |