FIG. 2.

Southern blot analysis of strains carrying deletion of the UPC2 gene. (A) Schematic view of the UPC2 gene and deleted alleles. The black rectangle corresponds to the probe used for Southern blot analysis, and the dotted rectangle corresponds to the UPC2 ORF. Deletions were performed as described in Materials and Methods. Expected lengths of ClaI fragments for wild-type and deleted alleles (upon looping out the URA3 marker by internal recombination) are also given. (B) Southern blot analysis of wild-type strain (WT) and upc2Δ1, upc2Δ2, upc2Δ3 andΔupc2 strains carrying deletion of one to four alleles of the UPC2 ORF, respectively. Lengths of fragments are given on the right part of the figure.