TABLE 1.
Strains used in this study
| Strain | Genotypea | Reference |
|---|---|---|
| SGY-243 (wild type) | ade2/ade2 Δura3::ADE2/Δ::ADE2 | 15, 23 |
| upc2Δ1 | UPC2/UPC2/UPC2/upc2Δ5-701::hisG | This study |
| upc2Δ2 | UPC2/UPC2/upc2Δ5-528::hisG/upc2Δ5-701::hisG | This study |
| upc2Δ3 | UPC2/upc2Δ4-335::hisG/upc2Δ5-334::hisG/upc2Δ5-701::hisG | This study |
| Δupc2 | upc2Δ4-178::hisG/upc2Δ5-177::hisG/upc2Δ4-528::hisG/upc2Δ5-701::hisG | This study |
| CSM2-2 | SGY-243 RP10::(pCaEXP) URA3 MET3 prom | This study |
| CSM2-3 | SGY-243 RP10::(pCaEXP) URA3 MET3 prom | This study |
| CSM1-2 | SGY-243 RP10::(pCaEXP) URA3 MET3 prom-UPC2 | This study |
| CSM1-3 | SGY-243 RP10::(pCaEXP) URA3 MET3 prom-UPC2 | This study |
| CSM13-1 | Δupc2 RP10::(pCaEXP) URA3 MET3 prom | This study |
| CSM13-2 | Δupc2 RP10::(pCaEXP) URA3 MET3 prom | This study |
| CSM15-1 | Δupc2 RP10::(pCaEXP) URA3 MET3 prom-UPC2 | This study |
| CSM15-2 | Δupc2 RP10::(pCaEXP) URA3 MET3 prom-UPC2 | This study |
a
prom, promoter.