FIG. 3.

Viability of S. aureus CI127 (A and C) and E. coli ORN103(pSH2) (B and D) incubated with CBHMC or HUVEC. Bacteria were added to a monolayer of CBHMC or HUVEC in antibiotic-free and serum-containing medium in wells of a 96-well tray at a ratio of 100:1. The mixture was incubated for 30 min, after which all unbound bacteria were removed and fresh medium was added. At different time points thereafter, the viability of residual bacteria was assessed by solubilizing the cells in each well with Triton X-100, and the number of viable bacteria was assessed by plating onto agar.