TABLE 1.
pfcrt allelic types and polymorphisms in microsatellite markers flanking pfcrt in P. falciparum samples collected from different districts/municipalities in the Philippines compared to those from other areas
| Origin (total no. of isolates) and sample | No. of isolates | % | MSb marker size (bp)
|
PFCRT (allelic type)a | MS marker size (bp)
|
|||
|---|---|---|---|---|---|---|---|---|
| B5M77 (−20 kb) | 2F10 (−5 kb) | PE12A (+6 kb) | 2H4 (+22 kb) | PE14F (+106 kb) | ||||
| Philippines | ||||||||
| Morong (20) | ||||||||
| PH1 group | 18 | 90 | 149 | 182 | CMNTHTYAQDLR (P2a) | 314 | 228 | 136 |
| PH2 | 1 | 5 | 149 | 182 | SMNTHTYAQDIR (P2b) | 314 | 228 | 136 |
| PH4 | 1 | 5 | 149 | 170 | CIETHALSESTI (E1a) | 314 | 204 | 145 |
| Bagac (4), B1 group | 4 | 100 | 149 | 182 | CMNTHTYAQDLR (P2a) | 314 | 228 | 136 |
| Dinalupiham (12) | ||||||||
| D1 group | 10 | 83 | 149 | 182 | CMNTHTYAQDLR (P2a) | 314 | 228 | 136 |
| D16 | 1 | 8 | 149 | 182 | CMNTHTYAQDLR (P2a) | 314 | 220 | 142 |
| D3 | 1 | 8 | 149 | 182 | SMNTHTYAQDIR (P2b) | 314 | 228 | 136 |
| Palawan (12) | ||||||||
| PAL2 group | 5 | 42 | 149 | 182 | SMNTHTYAQDIR (P2b) | 314 | 228 | 136 |
| PAL12 group | 2 | 17 | 149 | 182 | SMNTHTYAQDIR (P2b) | 314 | 228 | 151 |
| PAL7 | 1 | 8 | 149 | 182 | SMNTHTYAQDIR (P2b) | 314 | 228 | 148 |
| PAL6 | 1 | 8 | 149 | 182 | SMNTHTYAQDIR (P2b) | 314 | 220 | 136 |
| PAL5 group | 2 | 17 | 147 | 194 | SMNTHALSQDLR (P1a) | 328 | 228 | 136 |
| PAL1 | 1 | 8 | 147 | 194 | SMNTHALSQDLR (P1a) | 328 | 228 | 148 |
| Tayabas (6) | ||||||||
| T1 group | 2 | 33 | 149 | 174 | SMNTHALSQDLR (P1a) | 328 | 218 | 148 |
| T4 | 1 | 17 | 149 | 174 | SMNTHALSQDLR (P1a) | 328 | 184 | 148 |
| T2 group | 2 | 33 | 149 | 182 | SMNTHTYAQDIR (P2b) | 314 | 228 | 136 |
| T3 | 1 | 17 | 149 | 176 | CMNKHALAQNIR (CQS) | 328 | 182 | 142 |
| Mindanao (13) | ||||||||
| L104 group | 4 | 31 | 149 | 174 | SMNTHALSQDLR (P1a) | 328 | 184 | 148 |
| L088 group | 2 | 15 | 149 | 174 | SMNTHALSQDLR (P1a) | 328 | 184 | 151 |
| L016 | 1 | 8 | 149 | 174 | SMNTHALSQDLR (P1a) | 328 | 184 | 142 |
| M013 group | 2 | 15 | 149 | 174 | SMNTHALSQDLR (P1a) | 328 | 206 | 148 |
| M007 | 1 | 8 | 149 | 174 | SMNTHALSQDLR (P1a) | 328 | 186 | 148 |
| X039 | 1 | 8 | 149 | 174 | SMNTHALSQDLR (P1a) | 328 | 204 | 148 |
| X098 | 1 | 8 | 149 | 182 | SMNTHTYAQDIR (P2b) | 314 | 202 | 148 |
| X097 | 1 | 8 | 149 | 182 | SMNTHTYAQDIR (P2b) | 314 | 202 | 151 |
| Connor (10) | ||||||||
| C3 group | 7 | 70 | 149 | 174 | SMNTHALSQDLR (P1a) | 328 | 184 | 148 |
| C1 group | 3 | 30 | 149 | 174 | SMNTHALSQDLR (P1a) | 328 | 228 | 136 |
| Kabugao (5), Kab1 group | 5 | 100 | 149 | 174 | SMNTHALSQDLR (P1a) | 328 | 184 | 148 |
| Other areas | ||||||||
| Brazil (1), 7G8 | 1 | 151 | 190 | SMNTHALSQDLR (W1a) | 314 | 194 | 142 | |
| Thailand (5) | ||||||||
| Dd2 group | 2 | 149 | 170 | CIETHALSESTI (E1a) | 314 | 204 | 145 | |
| K1 | 1 | 149 | 170 | CIETHALSESII (E1b) | 314 | 204 | 145 | |
| TM93-C1088 | 1 | 149 | 170 | CIETLALSESTI (E1c) | 314 | 204 | 145 | |
| C2B | 1 | 149 | 170 | CIETHALSESTI (E1a) | 314 | 184 | 148 | |
| Solomon (4) | ||||||||
| N18 group | 2 | 149 | 174 | SMNTHALSQDLR (P1a) | 328 | 184 | 142 | |
| N70 group | 2 | 147 | 174 | SMNTHALSQDLR (P1a) | 328 | 184 | 142 | |
| PNG (6) | ||||||||
| AN001 group | 4 | 149 | 174 | SMNTHALSQDLR (P1a) | 328 | 184 | 142 | |
| AN018 group | 2 | 149 | 174 | SMNTHALSQDLR (P1a) | 328 | 192 | 139 | |