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. 2022 Sep 14;38(1):43–56. doi: 10.1007/s10557-022-07378-0

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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Amitriptyline enhanced the function of endothelial cells (a) Representative western blotting images and summarized data showing the effect of AMI (0–5 μM, 24 hours) on phosphorylated eNOS. (b) NO release from endothelial cells induced by AMI (0–5 μM, 24 h). (c) The effect of AMI on HUVECs proliferation. (d) Effects of AMI (0–5 μM, 24 h) on the viability of HUVECs. Bar: 400 μm in (c). Data are represented as the mean±SEM; All the experiments were performed independently three times. *P < 0.05, **P < 0.01, ***P < 0.001 vs control