Fig. 5.

Treatment with AMI improved tube formation and decreased oxidase-dependent ROS generation in HUVECs mediated by TNF-α (a) AMI alleviated the inhibitory role of TNF-α on angiogenesis in HUVECs. (b) Quantification of total branch. (c, d) Representative fluorescent images of DHE staining (c). Summary data (d) showing the roles of AMI in the production of ROS in HUVECs treated with TNF-α. Bar: 200 μm in (a), 400 μm in (c). The concentrate of TNF-α was 20 ng/ml. Data are represented as the mean±SEM (n = 5). All the experiments were performed independently three times. *P < 0.05, **P < 0.01, ***P < 0.001 vs control; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs TNF-α