Fig. 6.

Amitriptyline reversed TNF-α-induced increased MAPK and NF-κ B activity Western blotting and (a) statistical analysis (b) of expression of phosphor-NF-κ B, phosphor-ERK, phosphor-P38, and phosphor-JNK in TNF-α treated HUVEC. Data are represented as the mean±SEM (n = 3). (c–d) Western blotting data showing effects of AMI on expression levels of p-eNOS in TNF-α stimulated HUVEC. The concentrate of TNF-α was 20 ng/ml. All the experiments were performed independently three times. *P < 0.05, **P < 0.01, ***P < 0.001 vs control; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs TNF-α