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. 2023 Dec 26;130(4):555–567. doi: 10.1038/s41416-023-02530-5

Fig. 5. TGFβ is upregulated in brEC:melanoma co-cultures.

Fig. 5

a GSEA pathway analysis of D4M.3A co-cultured cells compared to SM1WT1 co-cultured cells; n = 4. b Volcano plot of selected EMT genes comparing D4M.3A co-cultured cells to SM1WT1 co-cultured cells; n = 4. TGFβ transcript levels of c D4M.3A:brECs co-cultures and d SM1WT1:brEC co-cultures; n = 4. TGFβ protein levels detected by Luminex in the supernatant of e D4M.3A:brECs co-cultures or f SM1WT1:brECs co-cultures; n = 4. g Western blot of D4M.3A, SM1WT1, or PTEN-RE cells treated with a time course of TGFβ and h quantification of pAKT/AKT ratio; n = 3. P values were determined by one-way ANOVA with Tukey’s multiple comparisons for each isoform in (c)–(f) and one-way ANOVA with Dunnett’s multiple comparisons test in (h).