PCR master mix for transgene investigation¹

Reagent	Amount
2× GoTaq G2 DNA polymerase mix	10 μL
10 μM forward primer (5′-KI-F or 3′-KI-R or control-F)	0.5 μL
10 μM reverse primer (5′-KI-R or 3′-KI-R or control-R)	0.5 μL
Genomic DNA	variable
Nuclease-free deionized water	to 20 μL
Total	20 μL

[Store Stock solution at 20°C, expiration date suggested by manufacture, the PCR reaction master mix must be freshly prepared]