FIG. 1.

Cytokine-specific anti-human MAbs (either FITC labeled or PE conjugated) were used in combination with allophycocyanin (APC)-labeled anti-CD4 MAb and peridinin chlorophyll (PerCP)-labeled anti-CD8 MAb for intracellular staining of cells within the lymphocyte scatter gate. The numbers in each quadrant represent the percentage of gated cytokine-producing cells within the CD4⁺- or CD8⁺-cell population. The dot plots from a representative child donor show that 46.8% of gated CD4⁺ cells were IL-2⁺–IFN-γ⁻, 5.3% were IL-2⁺–IFN-γ⁺, and 3.1% were IL-2⁻–IFN-γ⁺. Eleven percent of CD8⁺ cells produced IL-2⁺–IFN-γ⁻ cytokines, 2.3% produced IL-2⁺–IFN-γ⁺ cytokines, and 20% produced IL-2⁻–IFN-γ⁺ cytokines.