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. 2005 May;25(10):3956–3966. doi: 10.1128/MCB.25.10.3956-3966.2005

FIG. 1.

FIG. 1.

Mapping interaction of Ajuba and related LIM proteins with different PIPK enzymes. A to C. HEK 293 cells were cotransfected with control empty vector (−), Flag-Ajuba, Flag-PreLIM, or Flag-LIM regions of Ajuba and HA-tagged PIPK type Iα and Iβ (A), PIPK type IIβ and IIα (B), or PIPK type Iγ (C). Ajuba isoforms were immunoprecipitated with Flag antibodies, and bound products were Western blotted with HA antibodies (i.e., PIPK, upper panels) and Flag antibodies (i.e., Ajuba, lower panels). A fraction (5%) of each cell lysate was Western blotted for each protein (input). D. HEK 293 cells were cotransfected with HA-tagged PIPK enzymes and Myc-zyxin (upper two panels) or Myc-LPP (lower two panels). PIPKs were immunoprecipitated with HA antibodies (left), and bound products were Western blotted with Myc antibodies (i.e., LIM protein) and HA antibodies (i.e., PIPK enzyme). A fraction (5%) of each cell lysate was Western blotted for each protein (input).