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. 2005 May;25(10):4176–4188. doi: 10.1128/MCB.25.10.4176-4188.2005

FIG. 6.

FIG. 6.

Survival and proliferative pathways in melanoma maintenance. (A) Immunohistochemistry of phospho-ERK1/2 (p-ERK1/2) levels in regressing melanomas. The time (in hours) after doxycycline withdrawal is shown above the panels. (B) Immunoblot showing expression of PTEN in cultured mouse melanocytes (M) and in melanoma cell lines in vitro. (C) Immunoblots of lysates from regressing melanomas for levels of phospho-AKT (p-AKT), total AKT, Bcl-xl, cyclin D1, and p27. (D and E) Constitutive expression of Ereg partially sustains phospho-Akt (D) and Bcl-xl (E) levels. The time (in hours) after doxycycline withdrawal is shown above the lanes in panels C to E.