Table 1.

Calculations of protein concentrations and amounts of the proteins used in the proteasome labeling experiments

PI 01 group	Net A (562 nM)	Net A (562 nM) _repeat1	Net A (562 nM) _Average	Calculated con. (μg/mL)	Vol of lysate for 6 μg protein (μL)	Vol. Of reaction buffer (μL)
no wash	0.3087	0.3106	0.3097	2656	2.3	9.7
1X	0.3205	0.314	0.3173	2746	2.2	9.8
2X	0.3138	0.3072	0.3105	2661	2.3	9.7
3X	0.347	0.3468	0.3469	3116	1.9	10.1
4X	0.2899	0.2934	0.2917	2425	2.5	9.5