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| Reagent | Final concentration | Amount |
|---|---|---|
| Albumax II | 0.5% (w/v) | 5 g |
| RPMI 1640 | N/A | 10.5 g |
| Sodium bicarbonate | 12 mM | 8.06 g |
| Gentamicin | 20 mg/L | 4 mL |
| Hypoxanthine solution | 50 mg/L | .05 g |
| ddH2O | N/A | To 1 L |
| Total | N/A | 1 L |
Note: Ensure Albumax II has dissolved fully before adding other reagents. Adjust pH to 7.3 with sodium hydroxide. Filter sterilize and store at 4°C for up to 2 months. Warm to 37°C before use.
