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. 2024 Feb 2;35(1):102131. doi: 10.1016/j.omtn.2024.102131

Figure 4.

Figure 4

Evaluation of NCX1 expression in samples of ipsilateral cortex from ischemic rats treated with anti-miRNA103/107-loaded LNPs

(A) Expression analysis by real-time PCR of anti-miRNA103/107 levels in temporoparitel cortex of rats treated with anti-miR-103/107-loaded LNP1-4 24 h after i.v. injection. Results were normalized with respect to U6 snRNA as internal control. n = 3 samples per each group. (B) NCX1 protein levels are expressed as percentage vs. the sham-operated controls. Each column represents the mean ± SEM. Results of NCX1 expression were normalized with respect to α-tubulin. On the top, representative blots of NCX1 and α-tubulin signals in the sham and ischemic animals euthanized at 24 h from reperfusion. n = 4 samples per each group. ∗p < 0.05 vs. sham-operated control group. #p < 0.05 vs. vehicle group. (C) ncx1 mRNA levels are expressed as fold change of relative expression levels over the sham-operated group. Each column represents the mean ± SEM. Results of ncx1 expression were normalized with respect to GAPDH as internal control. n = 3 samples per each group. ∗p < 0.05 vs. control group.