Table 2.

Characterization of LNP-anti-miR103/107: size, polydispersity index (PI), zeta potential (ζ) encapsulation efficiency (EE%), and anti-miR103/107 actual loading

Formulation	Diameter (nm) ± SD	PI ± SD	ζ (mV ± SD)	Anti-miR103/107 actual loading (nmol/mg lipids ±SD)	EE (% ± SD)
LNP	147.7 ± 11.0	0.2 ± 0.01	−16.8 ± 3.7	–	–
LNP 1	154.3 ± 4.3	0.2 ± 0.03	−29.4 ± 3.5	20.5 ± 0.7	75 ± 2.3
LNP 2	147.4 ± 4.5	0.2 ± 0.03	−19.3 ± 2.6	34.7 ± 0.0	84 ± 2.5
LNP 3	173.4 ± 3.2	0.1 ± 0.04	−26.8 ± 2.4	22.3 ± 0.3	81 ± 3.1
LNP 4	153.3 ± 2.5	0.1 ± 0.02	−11.6 ± 3.2	38.1 ± 0.1	92 ± 2.8