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Distribution of fluorescently tagged 2μm reporter plasmid in ULP1 and ulp1ΔES yeast
| Genotype | 2μm-derived pSV5 plasmid distributiona (%)
|
||
|---|---|---|---|
| Equal | Unequal | Complete missegregation | |
| ULP1 [cir+] | 90 | 9 | <1 |
| ulp1ΔES [cir+] | 83 | 11 | 6 |
Cells with large buds and two well-separated equal DAPI-stained masses were scored for GFP-LacI repressor fluorescence as a measure of distribution of the Lac operator-containing 2μm-derived pSV5 plasmid. A total of 400 cells were scored for each strain.
