FIG. 3.

Targeted disruption of the NLRR4 gene. (a) Structures of the NLRR4 protein, cDNA, genomic locus, targeting vector, and mutated allele. Three boxes in the NLRR4 genome represent exons. The locations of the probes used for Southern blotting are shown (probes 1 and 2). E, EcoRI; B, BamHI; lacZ, Escherichia coli β-galactosidase cDNA; neo, neomycin resistance gene; HSV-tk, herpes simple virus thymidine kinase. (b) Identification of a homologous recombinant clone (A29) by southern blot analysis of BamHI- or EcoRI-digested genomic DNA from ES clones. (c) Southern blot analysis of BamHI-digested genomic DNA from mouse tails using probe 1. (d) Flow cytometric analysis using an NLRR4 antibody was performed on cells derived from wild-type and NLRR4^−/− embryos. NLRR4 protein was not detected in NLRR4^−/− cells.