KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER | |||
|---|---|---|---|---|---|
| Antibodies | |||||
| Mouse monoclonal anti-Tubulin (clone DM1A) | Sigma-Aldrich | Cat#T9026; RRID: AB_477593 | |||
| Chicken polyclonal anti-GFP | Novus Biologicals | Cat# NB100-1614-0.02ml | |||
| Mouse monoclonal anti-Abl2 (Ar11) | Hybridoma from Dr. Peter Davies, Albert Einstein Medical College, Bronx, NY | N/A | |||
| Goat anti-Chicken IgY (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 | Invitrogen | Cat#A32931 | |||
| Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ 647 | Invitrogen | Cat#A28181 | |||
| Bacterial and virus strains | |||||
| One Shot TOP10 Chemically Competent E. coli | Invitrogen | Cat#C404010 | |||
| BL21 Competent Cells | Agilent | Cat#200133 | |||
| DH10Bac Competent Cells | Invitrogen | Cat#10361012 | |||
| 3rd generation lentivirus produced in 293T cells to express eGFP, Abl2-eGFP, or Abl2Δ688-790-eGFP in COS-7 cells | This paper | N/A | |||
| Chemicals, peptides, and recombinant proteins | |||||
| Paclitaxel | Sigma-Aldrich | Cat#T7191 | |||
| Guanosine-5’-[(α,β)-methyleno]triphosphate, Sodium (GMPCPP) | Jena Bioscience | Cat#NU-405L | |||
| Nocodazole | Sigma-Aldrich | Cat#M1404–2mg; CAS: 31430-18-9 | |||
| Dextran from Leuconostoc spp. Mr ~70,000 | Sigma-Aldrich | Cat#31390–25G | |||
| Zeocin Selection Reagent | Gibco | Cat#R25005 | |||
| Deposited data | |||||
| cryoEM-map of microtubule only reconstruction | This paper | EMDB: EMD-41167 | |||
| cryoEM-map of microtubule-Abl2 reconstruction | This paper | EMDB: EMD-41169 | |||
| cryoEM-map of protofilament reconstruction of Abl2+MT | This paper | EMDB: EMD-41170 | |||
| cryoEM-map of protofilament reconstruction of Abl2+MT class 1 | This paper | EMDB: EMD-41173 | |||
| cryoEM-map of protofilament reconstruction of Abl2+MT class 2 | This paper | EMDB: EMD-41176 | |||
| cryoEM-map of protofilament reconstruction of Abl2+MT class 3 | This paper | EMDB: EMD-41177 | |||
| Experimental models: Cell lines | |||||
| Sf9 insect cells | ATCC | CRL-1711 | |||
| Hi Five insect cells | Thermo Fisher | B85502 | |||
| 293T cells | ATCC | CRL-3216 | |||
| COS-7 cells | ATCC | CRL-1651 | |||
| Abl2 KO COS-7 cells | Hu et al.38 | N/A | |||
| Abl2 KO COS-7 cells separately stably expressing eGFP, Abl2-eGFP, and Abl2Δ688-790-eGFP | This paper | N/A | |||
| Recombinant DNA | |||||
| FUGW for lentivirus generation | Lois et al.90 | Addgene#14883; RRID: Addgene_14883 | |||
| FUGW derived vector to stably express eGFP, Abl2-eGFP, or Abl2Δ688-790-eGFP | This paper | N/A | |||
| Abl2, Abl2-eGFP, Abl2 fragments, or Abl2 fragments-eGFP cloned into pFastBac1 | This paper | N/A | |||
| pGEX-6p1-GST-eGFP | This paper | N/A | |||
| pRK793 TEV S219V | The laboratory of Dr. Titus Boggon | N/A | |||
| pGEX-PP-Spastin(87–616)DeltaExon4 | Han et al. | Addgene#128794; RRID: Addgene_128794 | |||
| EB1-eGFP | The laboratory of Dr. Jonathon Howard | N/A | |||
| FUW-tau 2N4R | The laboratory of Dr. Elizabeth Rhoades | N/A | |||
| pHR-HNRNPA1C-mCh-Cry2WT | Shin et al.48 | Addgene #101226; RRID: Addgene_101226 | |||
| Software and algorithms | |||||
| ImageJ/Fiji | Schneider et al.91 | https://imagej.nih.gov/ij/ | |||
| EMBOSS | Madeira et al.92 | https://www.bioinformatics.nl/cgi-bin/emboss/ | |||
| PONDR-FIT | Xue et al.93 | https://www.disprot.org/ | |||
| Diso-Pred3 | Jones and Cozzetto et al.94 | http://bioinf.cs.ucl.ac.uk/psipred/ | |||
| Circular Dichroism algorithms (CONTIN, VARSLC, CDSSTR) | Provencher and Glöckner95; Compton and Johnson96; Sreerama and Woody97 | http://dichroweb.cryst.bbk.ac.uk/html/links.shtml | |||
| AlphaFold2 | Jumper et al.98 | https://www.deepmind.com/ | |||
| ChimeraX v1.4 | Pettersen et al.99 | https://www.rbvi.ucsf.edu/chimerax/ | |||
| Coot v.0.9.5 | Brown et al.100 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ | |||
| cryoSPARC v3.3.2 | Punjani et al101 | https://cryosparc.com/ | |||
| Protofilament refinement package | Debs et al.55 | https://gitlab.com/gedebs371/protofilament-refinement/-/tree/smooth_stable | |||
| SerialEM v.3.8.5 | Schorb et al.102 | https://bio3d.colorado.edu/SerialEM/ | |||
| RSEM v1.3.3 | Li and Dewey88 | https://deweylab.github.io/RSEM/ | |||
| STAR 2.7.11a | Dobin et al.103 | https://github.com/alexdobin/STAR | |||
| Matlab | The Mathworks, Inc. | https://nl.mathworks.com/products/matlab.html | |||
| Nikon Elements Software | Nikon | https://www.microscope.healthcare.nikon.com/products/software/nis-elements | |||
| Other | |||||
| Superdex 75 Increase 10/300 GL | Cytiva | Cat#29148721 | |||
| Superdex 200 Increase 10/300 GL | Cytiva | Cat#28990944 | |||
| Octet® NTA Biosensors | Sartorius | Cat#18–5101 | |||
| BLItz | ForteBio | https://intellicyt.com/products/fortebio/ | |||
| Carbon support Film 5–6nm on Square 400 mesh Cu grids | Electron Microscopy Sciences | Cat#CF400-Cu | |||
| R2/1, 300 mesh gold, QUANTIFOIL grids | Electron Microscopy Sciences | Cat#258376 | |||
| Nikon ECLIPSE Ti Series inverted microscope equipped with CFI Apo 100X TIRF 1.49 NA oil objective and Andor Zyla 4.2 sCMOS camera | Nikon, Andor Technology |
https://www.microscope.healthcare.nikon.com/products/inverted-microscopes/eclipse-ti-series; https://www.microscope.healthcare.nikon.com/products/optics/cfi-apochromat-tirf-series |
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| Nikon CSU-W1 SoRa spinning disk confocal microscope | Nikon | https://www.microscope.healthcare.nikon.com/products/confocal-microscopes/csu-series/csu-w1-sora | |||