FIG. 1.

Human TAF10 interacts with TAF3, TAF8, and SPT7L in vitro. (A) TAF3, TAF8, TAF10, and SPT7L were expressed individually or TAF10 was coexpressed with one of its potential partners (as indicated) in Sf9 cells by using the baculovirus expression system. Forty-eight hours after infection, WCEs were made, TAF10-associated proteins were subjected to IP with an anti-TAF10 mAb, the beads were extensively washed, boiled, and loaded onto an SDS-polyacrylamide gel, and TAF10-associated proteins were analyzed by Western blotting using the indicated antibodies. (B) TAF10 was first bound to protein G-Sepharose-anti-TAF10 antibody beads, the beads were extensively washed, and then protein extracts containing one of the three partners were incubated (as indicated) with the TAF10-containing beads (lanes 5 to 7) or with protein G-Sepharose antibody beads alone as controls (lanes 1 to 3) for 1 h at room temperature, and beads were extensively washed and bound proteins analyzed as described for panel A. IgG, immunoglobulin G (L, light chain; H, heavy chain).