FIG. 4.

Exogenously expressed TAF10 becomes exclusively nuclear when cells are cotransfected with one of TAF10's HF domain-containing interaction partners. The HF domain of TAF10 and the partner are required for nuclear localization. (A to E) HeLa cells were cotransfected with the indicated CFP- and YFP-containing expression vectors, and localization of the expressed proteins was visualized by fluorescence microscopy. Flag-TAF3 was visualized by immunofluorescence. In panel A, pYFP-TAF10-transfected cells were incubated for 4 h with 20 ng/ml leptomycin B. The images shown in each panel are representative of all the transfected cells.