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. 2005 May;25(10):4092–4104. doi: 10.1128/MCB.25.10.4092-4104.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 6. — YFP-TAF10 diffuses freely within the cytoplasm but binds to structures in the nucleus. (A) Quantitative FRAP analysis of YFP, YFP-TAF10 in the cytoplasm, nuclear YFP-TAF10-NLS^TAF8, and nuclear YFP-TAF10 after coexpression with TAF8. YFP and cytoplasmic YFP-TAF10 show similar, very fast recovery kinetics in the cytoplasm (250 ms), whereas nuclear YFP-TAF10 shows much slower recovery kinetics (3 s) than its cytoplasmic counterpart. (B) Confocal series of pictures of transfected cells before and after photobleaching of the nuclear fluorescent pool of either YFP or YFP-TAF10. YFP shows fast and almost complete recovery within the first 5 min after bleaching. YFP-TAF10 exhibits only minimal recovery even after 30 min.