FIG. 7.

In vitro TAF10 binding to importin β is dependent on the coexpression of either TAF8 or TAF3, but not SPT7L. (A to F) Six-His-tagged GST, six-His-tagged importin α, and six-His-tagged importin β were expressed in Escherichia coli and bound to Ni-NTA beads that were then extensively washed. HeLa cells were cotransfected with CFP- and YFP- or Flag-containing expression vectors (as indicated at the top of each panel); 48 h after transfection, WCEs were prepared and the extracts were incubated with the control His-tagged GST- as well as the importin α- and importin β-containing beads. Beads were then washed and bound proteins analyzed by Western blotting with an anti-GFP antibody. A small amount (0.1%) of the input extract was also analyzed on the same blots.