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. 2023 Nov 30;39(1):81–96. doi: 10.1016/j.virs.2023.11.010

Fig. 6.

Fig. 6

Oseltamivir (OST) inhibits the expression of HIF-1α during H1N1 virus infection. A HE staining of lung tissue from H1N1-infected mice (n ​= ​5, 1000 ​pfu per mouse, Days 3 and 5) treated with or without a low (L) (OST, 30 ​mg/kg, intragastric, once every 12 ​h for Days 3 and 5) or high (H) (OST, 60 ​mg/kg, intragastric, once every 12 ​h for Days 3 and 5) dose of oseltamivir. Scale bar ​= ​100 ​μm. B, C The concentrations of IL-1β, IL-6 and TNF-α in serum and BALF from H1N1-infected mice (n ​= ​5, 1000 ​pfu per mouse) after treatment with or without oseltamivir were measured by ELISA. D The distribution of HIF-1α protein in A549 ​cells after OST treatment (0.75 ​μg/mL for 48 ​h) was investigated by immunofluorescence staining (scale bar ​= ​50 ​μm). E, F The protein expression level of HIF-1α in mouse lung tissues (n ​= ​5, 1000 ​pfu per mouse for Days 3 and 5) and A549 ​cells (MOI ​= ​1 for 24 ​h) after OST treatment was measured by Western blotting. β-Actin served as the loading control. The data are presented as the means with SDs. Statistical analysis was performed by one-way ANOVA. ∗P ​< ​0.05.