Table 1. Bacterial Strains Used Throughout This Study.

name	relevant features	purpose	reference
E. coli MG1655	K-12 F– λ–	recipient of Golden Gate library transformation for subsequent cPCR amplicon sequencing	(18)
E. coli BL21-CodonPlus (DE3)-RIL	F–ompT hsdS(rB–mB–) dcm+ TetRgal λ(DE3) endA Hte [argU ileY leuW CamR]	overexpression and purification of Tn5R27S,E54K,L372P from Henning et al.	Agilent