Table 2. Oligonucleotides Used in This Study.
| ID | sequence (5′ → 3′)a | purpose |
|---|---|---|
| SLo0100 | 5′[phos] CTGTCTCTTATACACATCT | Tn5-ME reverse for transposase loading; Henning et al. |
| SLo0151 | CCCAGTCACGACGTTGTAAAACG | M13 forward primer serving as control primer |
| SLo0152 | AGCGGATAACAATTTCACACAGG | M13 reverse primer serving as control primer |
| SLo0673 | CCCAGTCACGACGTTGTAAAACGAGATGTGTATAAGAGACAG | Tn5-ME with added M13 forward sequence |
| SLo0674 | AGCGGATAACAATTTCACACAGGAGATGTGTATAAGAGACAG | Tn5-ME with added M13 reverse sequence |
| SLo1577 | CCCAGTCACGACGTTGTAAAACGCGTCAATTGTCTGATTCGTTACCA | forward primer for colony PCR amplification with added M13 forward sequence |
| SLo1578 | AGCGGATAACAATTTCACACAGGCTTCTCTCATCCGCCAAAACA | reverse primer for colony PCR amplification with added M13 reverse sequence |
a
Sequence hybridizing is underlined, and attached sequences are in italics.