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. 2005 Apr 28;33(8):2384–2394. doi: 10.1093/nar/gki534

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Native gel retardation assay between labeled pK1 and purified SmpB-His from A.aeolicus. (A) Increasing amounts of SmpB was incubated with pK1; a full-length tmRNA–SmpB complex is shown as a positive control; (B) a 3- to 10-fold excess of cold PK1 competed labeled PK1 out of the complex. C: Negative control.