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. 2005 Mar;4(3):526–535. doi: 10.1128/EC.4.3.526-535.2005

FIG. 4.

FIG. 4.

Sxi1α and Sxi2a regulate pheromone gene expression. A Northern blot showing the levels of selected transcripts in wild-type, sxi1αΔ, and sxi2aΔ strains after growth on V8 medium in three different contexts, indicated below the lane numbers: haploid strains alone, mixed mating cocultures, and diploid strains alone. The top two panels show RNA probed with the MFa and MFα ORFs. The third panel shows the same blot probed with portions of the SXI1α and SXI2a ORFs. The bottom panel shows the same blot probed with a portion of the GPD1 ORF. Lane 1, wild-type haploid a; lane 2, wild-type haploid α; lane 3, haploid sxi1αΔ; lane 4, haploid sxi2aΔ; lane 5, wild-type a cocultured with wild-type α; lane 6, wild-type a cocultured with sxi1αΔ; lane 7, sxi2aΔ cocultured with a wild-type α; lane 8, sxi2aΔ cocultured with a sxi1αΔ; lane 9, a/α diploid; lane 10, a/sxi1αΔ diploid; lane 11, sxi2aΔ/α diploid; lane 12, sxi2aΔ/sxi1αΔ diploid.