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. 2005 Mar;4(3):526–535. doi: 10.1128/EC.4.3.526-535.2005

FIG. 5.

FIG. 5.

Sxi1α and Sxi2a interact with one other. The top panel in each set (−leu −trp) confirms the presence of each two-hybrid plasmid under selection. The middle and bottom panels indicate the presence or absence of activation of a biosynthetic reporter gene (either ADE2 or HIS3). The presence or absence of indicated constructs is represented with + or − over each panel. The fusion proteins tested are indicated in schematic form: the hatched box BD represents the Gal4 DNA BD, the speckled box AD represents the Gal4 AD, and the black box represents the Sxi2a homeodomain region. Growth in a test spot indicates activation of the reporter. (A) Lanes 1 to 4 reveal a transactivating property of Sxi2a in the absence of Sxi1α when fused to a DNA BD. (B) Lanes 5 to 11 confirm an interaction between Sxi1α and Sxi2a and define the region of Sxi2a necessary for interaction. The results of liquid β-galactosidase assays in each column are shown in Miller units.