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Chromatin silencing gene mutants affect PIS1-cat expression. A PIS1 promoter fragment (pPIS-325) was fused to the cat reporter gene and integrated in single copy at the GAL4 locus in strains BY4742 (MATα) and BY4741 (MATa) and isogenic rif1Δ, hst3Δ, and hst4Δ strains. PIS1-cat expression was assayed in transformants grown in medium containing either 2% glucose (MATα, solid bars; MATa, hatched bars) or 3% glycerol (MATα, empty bars; MATa, horizontal bars). WT, wild type.
