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. 2005 Mar;4(3):604–614. doi: 10.1128/EC.4.3.604-614.2005

FIG. 9.

FIG. 9.

A. PIS1-cat expression is not regulated by growth phase or hyperosmotic stress. BRS1001 transformants containing pPIS1-918 were grown in Ura− synthetic medium in the presence of inositol and choline (I+C+) (solid squares), in the absence of inositol and choline (I−C−) (open squares), in the presence of 0.7 M NaCl (solid circles), or in the presence of inositol, choline, and 0.7 M NaCl (open circles). Yeast extracts from the transformants were prepared and assayed for CAT activity. B. Growth patterns of cultures used as described above (A).