Figure 2.
Analysis of cell adherence during washing steps
(A) Experimental steps for testing cell adherence during plate washing (left). Images of HEK293T or HeLa cell layers after staining with crystal violet and washing with an ELISA washer.
(B) HeLa cells 48 h post-transfection with 0.3 μg plasmid DNA (ptANCHOR-CD82-Wuhan-mCherry) per well of a 96-well plate. Cell layers were fixed using 2% paraformaldehyde (PFA) as a fixative when tested for cell adherence during washing steps. Scale bars, 100 μm.