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. 2024 Feb 5;27(3):109123. doi: 10.1016/j.isci.2024.109123

Figure 4.

Figure 4

Binding of ACE21-740-V5-His on various SARS-CoV-2 RBD variants

(A) Representative confocal laser scanning microscopy (CLSM) images of immunostained ACE21-740-V5-His protein (green) bound on transfected HeLa cells expressing tANCHORed RBD protein (red); scale bars: 10 μm. Mock control contains transfected HeLa cells without plasmid DNA and incubated with ACE21-740-V5-His protein in parallel. Images were merged and show nuclei stained with Hoechst 33342 (blue), DIC (differential interference contrast), and fluorescence channels.

(B) Violin plots of the ACE21-740-V5-His protein binding to RBD variants (n = 8). The V5 control (HeLa cells transfected with ptANCHOR-CD82-V5-His-mCherry) was not incubated with a supernatant containing secreted ACE21-740-V5-His protein. The p values were calculated by an unpaired, two-tailed Student’s t test; non-significant (ns): >0.05; ∗p < 0.05, ∗∗∗∗p < 0.0001.