FIG.1.
Cdc24 and Cdc42 levels are reduced in cdc24Δ/PMETCDC24 and cdc42Δ/PMETCDC42 strains. (A) Transcript levels of CDC24 and CDC42 in the wild type and ectopic expression mutants. RNA transcript levels normalized to actin transcript levels (2Ctgene/2Ctactin) were determined from budding cells grown at 30°C in YEPD-Uri medium. Values are the means of three or four independent experiments, with bars representing standard deviations. (B) Cdc42 protein levels in the wild type and ectopic expression mutants. Immunoblot analysis of Cdc42 protein levels from the indicated strains grown in YEPD-Uri medium at 30°C. Anti-Cdc42 polyclonal antibodies were used. The upper band is a nonspecific protein cross-reacting with anti-Cdc42 antibody.